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Could Not Calculate Ungapped Karlin-altschul Parameters Due To

But I get this error messag... BLASTp query/options error Hello everyone, I am performing standalone blastp, I already used these commands on windows but ... Given that your sequences are highly repetitive, this is likely if you have some filtering turned on, which I seem to remember blastall does by default. I am struggling to want using Socrates. (http://bioinf.wehi.edu.au/socrates/) I looked  up ... have a peek at this web-site

Please don't refer me to the similar thread on biostar, I see it, but not useful for me. Please verify the query sequence(s) and/or filtering options snayfach commented Sep 8, 2015 This issue is now fixed. I wrote ... Check the database to make sure it only has valid amino-acid characters. https://www.biostars.org/p/167725/

Thanks, ¬† blast • 3.5k views ADD COMMENT • link • Not following Follow via messages Follow via email Do not follow modified 17 months ago by Ginsea Chen • 70 can't get staxids with blastn megablast against nt Hi everyone, Please I really need help. Blast Gives Cryptic Errors I have a list of proteins in fasta format (say goodProteins.fasta).

Contact Us - SEQanswers Home - Archive - Top Powered by vBulletin Version 3.8.9Copyright ©2000 - 2016, vBulletin Solutions, Inc. Similar posts • Search » getting error in runing tblastn Hello group, i am trying to run tblastn of version 2.2.26+ for mapping peptides on eukaryotic ge... vBulletin ©2000 - 2016, Jelsoft Enterprises Ltd. I followed th...

Reload to refresh your session. I would like to perform a tblastn w... User contributions on this site are licensed under the Creative Commons Attribution Share Alike 4.0 International License. If it is other way around, BLASTX is used to fulfill the purpose.¬† ¬† ~Prakki Rama. ¬† ADD COMMENT • link modified 2.6 years ago • written 2.6 years ago by

URL: Next message: [protocols] blastall error "Could not calculate ungapped Karlin-Altschul parameters" Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] More information about Please verify the query sequence(s) and/or filtering options I call blast like this: blastall -p blastp -i MaulwurfLeber_prots.fasta -d Pool_new_unclustered -o Contigs_prots_vs_New_unclustered.tab -a 8 -m 8 -e 0.001 The reads which converting a fasta database to blastp database hello im runnnig a wwwblast software on my server i do have a fasta database and i would like to ... My AccountSearchMapsYouTubePlayNewsGmailDriveCalendarGoogle+TranslatePhotosMoreShoppingWalletFinanceDocsBooksBloggerContactsHangoutsEven more from GoogleSign inHidden fieldsSearch for groups or messages To use Google Groups Discussions, please enable JavaScript in your browser settings, and then refresh this page. .

Password Register FAQ Community Calendar Today's Posts Search You are currently viewing the SEQanswers forums as a guest, which limits your access. http://lists.idyll.org/pipermail/protocols/2014-August/000025.html Mothur_chimera.uchime running error Please any body help me. But whe... Sign in to comment Contact GitHub API Training Shop Blog About © 2016 GitHub, Inc.

ADD REPLY • link written 2.6 years ago by Pierre Lindenbaum ♦ 87k Guessing from your file naming, the query is nucl and the DB is protein. Check This Out myposts blastp blast fasta • 1.0k views ADD COMMENT • link • Not following Follow via messages Follow via email Do not follow modified 13 months ago • written 13 months Problem Running Blast Jobs. Personal Open source Business Explore Sign up Sign in Pricing Blog Support Search GitHub This repository Watch 1 Star 1 Fork 0 lmrodriguezr/Polloc Code Issues 7 Pull requests 0 Projects

I'm running standalone blast locally. blast two DNA sequence which were repeat-masked How to blast two DNA sequence that were repeat-masked using blastn. My blast query sequencess are in a fasta file called human... http://awendigital.com/could-not/could-not-calculate-ungapped-karlin-altschul-parameters.html I am interested in using Mothur for metagenomics analysis.

How To Get Top Hits? Powered by Biostar version 2.3.0 Traffic: 418 users visited in the last hour Latest Open RNA-Seq ChIP-Seq SNP Assembly Tutorials Tools Jobs Forum Planet All » View Posts Latest Open Ncbi Legacy Blast Usage With Tblastn/Pssm I'm trying to get a webservice for protein discovery running.

ADD REPLY • link written 2.6 years ago by Ram ♦ 10k 3 2.6 years ago by Prakki Rama • 1.9k Singapore Prakki Rama • 1.9k wrote: The option¬†"-F"¬† for masking

about • faq • rss Community Log In Sign Up Add New Post Question: blast error invalid query/ sequence/ filtering options. i am getting the following error. My... However, remember that an empty result file without any fatal error message could also mean that there are no results to be had.

RSeQC (infer_experiment) problem HI everyone I am using RSeQC to determine strandness.  When running the command infer_experiment... You are not using the latest blast so if possible upgrade. Reload to refresh your session. have a peek here The following warning is now reported for each sequence that consists of all Ns: [ScoreBlkKbpUngappedCalc] Warning: Could not calculate ungapped Karlin-Altschul parameters due to an invalid query sequence.

Tblastx Exception Error Hi, I am having some problems running tblastx locally on my HPC. Warning message in findOverlap function in R I am trying to find overlap between chicken (galGal4) repeat masker file (.rmsk), and promoter re... maubp View Public Profile Send a private message to maubp Visit maubp's homepage! Please verify the query sequence(s) and/or filtering options" kindly tell me how to solve this problem?

Powered by Biostar version 2.3.0 Traffic: 418 users visited in the last hour Latest Open RNA-Seq ChIP-Seq SNP Assembly Tutorials Tools Jobs Forum Planet All » View Posts Latest Open i ahve also run by keeping filtering turn off with the following command tblastn -query sequence.fa -out output.txt -F T -subject ref_protein.fa -evalue 1e-5 -outfmt 7 -max_target_seqs 5 -best_hit_score_edge 0.05 -best_hit_overhang